Journal: PLoS Pathogens

Article Title: EDP-938, a novel nucleoprotein inhibitor of respiratory syncytial virus, demonstrates potent antiviral activities in vitro and in a non-human primate model

doi: 10.1371/journal.ppat.1009428

Figure Lengend Snippet: In vitro activity of EDP-938 against RSV laboratory strains.

Article Snippet: Maintained in ATCC Airway Epithelial Cell Basal Medium with HLL Supplement, L-glutamine, Extract P, Airway Epithelial Cell Supplement or PromoCell Airway Epithelial Cell Growth Medium with Supplement Mix and 1% P/S respectively.

Techniques: In Vitro, Activity Assay, Virus

Journal: Advanced Science

Article Title: A General High‐Throughput Mucus Microrheology Platform for Quantitative and Scalable Mucus Phenotyping

doi: 10.1002/advs.77750

Figure Lengend Snippet: Donor information for primary airway epithelial cultures. Donor identifiers are reported as used throughout the manuscript and figures.

Article Snippet: Enriched progenitor cells were seeded to 12‐mm transwell (Corning) and cultured in small airway epithelial growth medium (Promo Cell) supplemented with 0.5 μ M A–83–01 (Tocris), 0.5 μ M DMH–1 (Tocris) and 5 μ M ROCK Inhibitor Y27632 for four days.

Techniques: Control

Journal: Journal of Virology

Article Title: Resistance of endothelial cells to SARS-CoV-2 infection in vitro

doi: 10.1128/jvi.01205-25

Figure Lengend Snippet: SARS-CoV-2 virus infection in human airway epithelial cells in air:liquid interface, Vero E6, and endothelial cells. Human airway epithelial cells grown in an air:liquid interface (MucilAir) were infected with SARS-CoV-2 live virus (MOI = 0.1). Infectious virus released to the apical side of the epithelium was determined over time (6, 24, 48, and 72 h post-infection) ( A ). In separate studies, the levels of SARS-CoV-2 nucleocapsid or spike protein in Vero E6 and endothelial cells (treated with media only [untreated] or IL-1β [10 ng/mL; 3 h]) at 24 ( B ) and 72 ( C ) h post-infection with SARS-CoV-2 (MOI = 0.1) were determined using fluorescent imaging. Mock controls (media only) experiments were run simultaneously using each endothelial cell line. Data are shown as n = 3 (pooled donors) for Mucilair cells ( A ) and n = 3 (separate donors) for human aortic (AoEC), lung microvascular (HMVEC), and blood outgrowth endothelial cells (BOECs). Data are shown as mean ± SEM for (A) and representative images shown for (B) and (C) (scale bar = 25 µm).

Article Snippet: Nasal epithelial cells (Promocell) were maintained in Airway Epithelial Growth Media (Promocell) and differentiated nasal epithelial cells (MucilAir) (Epithelix, Switzerland) were grown in air-liquid interface culture using MucilAir Culture Medium (Epithelix).

Techniques: Virus, Infection, Imaging

Journal: Journal of Virology

Article Title: Resistance of endothelial cells to SARS-CoV-2 infection in vitro

doi: 10.1128/jvi.01205-25

Figure Lengend Snippet: SARS-CoV-2 virus infection in human airway epithelial cells in air:liquid interface, Vero E6, and endothelial cells. Human airway epithelial cells grown in an air:liquid interface (MucilAir) were infected with SARS-CoV-2 live virus (MOI = 0.1). Infectious virus released to the apical side of the epithelium was determined over time (6, 24, 48, and 72 h post-infection) ( A ). In separate studies, the levels of SARS-CoV-2 nucleocapsid or spike protein in Vero E6 and endothelial cells (treated with media only [untreated] or IL-1β [10 ng/mL; 3 h]) at 24 ( B ) and 72 ( C ) h post-infection with SARS-CoV-2 (MOI = 0.1) were determined using fluorescent imaging. Mock controls (media only) experiments were run simultaneously using each endothelial cell line. Data are shown as n = 3 (pooled donors) for Mucilair cells ( A ) and n = 3 (separate donors) for human aortic (AoEC), lung microvascular (HMVEC), and blood outgrowth endothelial cells (BOECs). Data are shown as mean ± SEM for (A) and representative images shown for (B) and (C) (scale bar = 25 µm).

Article Snippet: Nasal epithelial cells (Promocell) were maintained in Airway Epithelial Growth Media (Promocell) and differentiated nasal epithelial cells (MucilAir) (Epithelix, Switzerland) were grown in air-liquid interface culture using MucilAir Culture Medium (Epithelix).

Techniques: Virus, Infection, Imaging